Mating type Zygosity and Virulence in a Human Pathogenic Fungus Candida Albicans
Mating-type zygosity and virulence in a human pathogenic fungus, candida albicans by Wei Wu Candida albicans is an obligate diploid fungal pathogen of humans, and neither a complete sexual life cycle nor meiosis has been described. The majority of natural C. albicans strains is heterozygous .... Published date on: 2007 with total page: 183 pages. Publisher of Mating-type Zygosity and Virulence in a Human Pathogenic Fungus, Candida Albicans is ProQuest.
Candida albicans is an obligate diploid fungal pathogen of humans, and neither a complete sexual life cycle nor meiosis has been described. The majority of natural C. albicans strains is heterozygous (a/alpha) at the mating type locus (MTL), and must undergo MTL-homozygosis to mate. The research described in this thesis focuses on the mechanisms of MTL-homozygosis and the role of the MTL locus in virulence and pathogenesis. To understand how MTL-homozygosity was achieved, polymorphic markers on chromosome 5 were analyzed in the spontaneously derived and natural MTL-homozgyous strains. It is demonstrated that chromosome loss followed by duplication of the retained homolog (uniparental disomy) is the most common mechanism in the spontaneous generation of MTL-homozygotes in vitro, whereas natural MTL-homozygous strains undergo mitotic recombination to achieve MTL-homozygosis. To understand how the mating type locus contributes to virulence, a murine model for systemic infection was employed to compare the virulence of strains that differ in zygosity of the MTL locus and genes on chromosome 5. Natural a/alpha strains of C. albicans are more virulent and more competitive than their spontaneous MTL -homozygous offspring. Deletion of either the a or alpha copy of the MTL locus of natural a/alpha strains results in a small decrease in virulence, and a small decrease in competitiveness. Loss of heterozygosity of non-MTL genes along chromosome 5, however, results in larger decreases in virulence and competitiveness. Natural MTL-homozygous strains are on average less virulent than natural MTL-heterozygous strains and arise by multiple mitotic recombinations along chromosome 5 outside of the MTL region. These results are consistent with the hypothesis that a competitive advantage of natural a/alpha strains over MTL-homozygous offspring maintains the mating system of C. albicans.
Do you have read this ebook mating type zygosity and virulence in a human pathogenic fungus candida albicans? If you have read an ebook before don't be hasitate to leave review about the book mating type zygosity and virulence in a human pathogenic fungus candida albicans. But otherwise, you can registar/sign up before downloding this pdf book. We have huge database of ebooks in our library. If you bought an ebook than you want another ebook we suggest you to become our library member.
What do you get from becoming member in our library? 1.You can read free a lot of books from our database. 2.You can use any kind of ebook reader available on market, because our book available in various format. 3.You able to find your ebook fastly through our library because we put ebooks based on categories such as adventure, art, law, social, health, medical, science, romance, fiction, non-fiction and etc.